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Blechschmidt collectionスキャンプロジェクトについての論文がCongenital anomaliesに掲載されました。先天異常標本解析センター、ドイツ・ゲッティンゲン大学解剖学教室との共同プロジェクトです。
（ゲッティンゲン大学ヒト胚子コレクションの組織学的解析およびデジタルアトラス作成；基盤(B)(海外 )2015-2017)
- Blechschmidt コレクションの組織標本を、市販のフラットベッド スキャナーを使用して4800 dpiの解像度画像にデジタル化
- CRL64mm, CRL17.5mm (CS20) の2 つの標本について、最新の技術を使用して、立体再構成
⑦ Miyazaki R, Makishima H, Männer J, Sydow HG, Uwabe C, Takakuwa T, Viebahn C, Yamada S. The Blechschmidt Collection: revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques, Congenit Anom, 2018, 58, 152-157, doi: 10.1111/cga.12261
ABSTRACT
Along with the Carnegie Collection in the United States and the Kyoto Collection in Japan, the Blechschmidt Collection (Georg-August-University of Göttingen, Germany) is a major historical human embryo and fetus collection. These collections are of enormous value to human embryology; however, due to the nature of the historical histological specimens, some stains are fading in color, and some glass slides are deteriorating over time. To protect these specimens against such degradation and ensure their future usefulness, we tried to apply modern image scanning and computational reconstruction. Samples of histological specimens of the Blechschmidt Collection were digitized into images using commercial flatbed scanners with a resolution of 4800 pixels per inch. Two specimens were reconstructed into three-dimensional (3D) images by using modern techniques to vertically stack two-dimensional images of the slices into 3D blocks. The larger specimen of crown-rump length (CRL) 64.0 mm, a series of very large histological sections in human embryology, was reconstructed clearly, with its central nervous system segmented before stacking. The smaller specimen of CRL 17.5 mm was also reconstructed into 3D images. The outer surface of the embryo was intact, and its development was classified according to the widely used Carnegie stages (CSs). The CS of the specimen was identified as the later half of CS 20. The invaluable Blechschmidt Collection can be revisited for further research with modern techniques such as digital image scanning and computational 3D reconstruction.
古市さんの卒論がAnat Recに掲載されました。
連続組織切片の 3-D 再構成を行い、胚子期末のWillis 輪( CW )の形成を検討しました。
- CW は胚子期末のすべての標本で閉鎖、
- CWは単一の平面でなく、複数の階段状の平面で構成
- 中脳と間脳の屈曲を反映してCW は尾部で急激に湾曲
- 85%(17/20)でvariation観察
- （anterior partのみ10例、anterior+posterior 6例、posteriorのみ１例）
- 観察されたvariationは、胎児、新生児、成人で報告されているものと同様
- CWのvariationが開始時期から存在することを示唆
33. Furuichi K, Ishikawa A, Uwabe C, Makishima H, Yamada S, Takakuwa T,
Variations of the circle of Willis at the end of the human embryonic period, 2018, 301, 1312-1319, doi:10.1002/ar.23794
ABSTRACT
Variations of the circle of Willis (CW) influence blood supply to the brain and adjacent structures in adults. We examined the formation of the CW in 20 human embryo samples at the end of the embryonic period using 3-D reconstructions of serial histological sections. The CW was closed in all samples, and did not form in a single plane, but was composed of multiple stair-like planes. The artery acutely curved at the caudal part of the CW, namely, at the inlet of the basilar artery and bifurcation of the P1 segment of the posterior cerebral artery (PCA), reflecting flexure of the mesencephalon and diencephalon at this stage. Variations were observed in 17 of 20 samples—only anterior parts (anterior communicating artery [Acom] and anterior cerebral artery [ACA]) in 10 samples, only posterior parts (posterior communicating artery [Pcom]) in one sample, and both anterior and posterior parts in six samples. Variations included the Acom formed as partially duplicated in three samples, duplicated in four, plexiform in three, and no channel as a result of a single azygos ACA in one. The ACA formed as duplicated in two, median ACA in two, and right hypoplasia in one. The Pcom formed in hypoplasia of either side in six samples. Variations observed in this study are similar to those observed in fetuses, neonates, and adults. The P1 segment of PCA was very large in all samples. The present observations indicate that variations in the CW are present from the initiation of CW formation.
 第58回日本先天異常学会で発表しました（7/27−7/29, 東京）
台風も近づき慌ただしい学会でした。
..
奨励賞受賞講演
・金橋 徹、山田 重人、田中 美玲、廣瀬あゆみ、上部千賀子、巨瀬 勝美、米山 明男、武田 徹、高桑 徹也
:大規模コレクションから潜在的な異常例を明らかにする新手法
 「多元計算解剖学」第4回サマーワークショップ(7月14、15日、神戸市)に参加しました。新学術領域研究「医用画像に基づく計算解剖学の多元化と高度知能化診断・治療への展開」研究班のmeetingです。今年度最終年になりました。
  佐賀県立九州シンクロトロン光研究センターに行きました。(2018.0612
位相CT撮像データからイメージ作成を行う手法を共同研究者の米山先生から習いました。
先天研開設40周年記念号がAnatomical Recに掲載されました。
先天研開設40周年記念号；ヒト胚子・胎児研究についての総説 Anat Rec 2018, 301,960-969 (クリックで拡大されます）
 4/2から約3ヶ月間クララ−ムネスコさんがフランスのモンテペリエから胚子研究に来られました。胚子期の顔面形成について解析をする予定です。
金橋君が2018年度日本先天異常学会奨励賞を受賞しました。おめでとうございます。
Kanahashi T, Yamada S, Tanaka M, Hirose A, Uwabe C, Kose K, Yoneyama A, Takeda T, Takakuwa T, A novel strategy to reveal the latent abnormalities in human embryonic stages from a large embryo collection, Anatomical Record, 299,8-24,2016 10.1002/ar.23281(概要), *299(1),2016の表紙に採用されました。DOI: 10.1002/ar.23206 (cover page)
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