本研究室で行われているヒト胚子・胎児研究についてまとめた総説がAnatomical Recに掲載されました。
幅広い発達期間をカバーする大規模で高解像度なデジタルデータセットの確立し、そのメリットを活かした研究展開を示しました。デジタルデータのメリット、それを活かした展開として
- 形態計測分析に適した平面の抽出、
- 形態学的観察の効率化。
- 発生の特徴を定量的に評価、実証、異常なサンプルのスクリーニング
- 超音波検査データを比較
- 解剖学的ランドマークの 3D 座標は
- 位置変化とそれらの関係を分析するために有用
- 3D 座標を使用した微分成長の説明
- 統計分析、数学的分析に応用
器官形成時の動的で複雑なプロセスの解析、正確な形態学的データの提供への寄与が期待されます。
30. Takakuwa T, 3D analysis of human embryos and fetuses using digitized datasets from the Kyoto Collection, Anat Rec 2018, 301,960-969 doi: 10.1002/ar.23784 (英文で読む）
ABSTRACT
Three-dimensional (3D) analysis of the human embryonic and early-fetal period has been performed using digitized datasets obtained from the Kyoto Collection, in which the digital datasets play a primary role in research. Datasets include magnetic resonance imaging (MRI) acquired with 1.5 T, 2.35 T, and 7 T magnet systems, phase-contrast X-ray computed tomography (CT), and digitized histological serial sections. Large, high-resolution datasets covering a broad range of developmental periods obtained with various methods of acquisition are key elements for the studies. The digital data have gross merits that enabled us to develop various analysis. Digital data analysis accelerated the speed of morphological observations using precise and improved methods by providing a suitable plane for a morphometric analysis from staged human embryos. Morphometric data are useful for quantitatively evaluating and demonstrating the features of development and for screening abnormal samples, which may be suggestive in the pathogenesis of congenital malformations. Morphometric data are also valuable for comparing sonographic data in a process known as “sonoembryology.” The 3D coordinates of anatomical landmarks may be useful tools for analyzing the positional change of interesting landmarks and their relationships during development. Several dynamic events could be explained by differential growth using 3D coordinates. Moreover, 3D coordinates can be utilized in mathematical analysis as well as statistical analysis. The 3D analysis in our study may serve to provide accurate morphologic data, including the dynamics of embryonic structures related to developmental stages, which is required for insights into the dynamic and complex processes occurring during organogenesis. Anat Rec, 301:960–969, 2018.